27-Hydroxycholesterol induces the transition of MCF7 cells into a mesenchymal phenotype

descriptionPublicationkeyboard_double_arrow_right Article 28 Apr 2011 Chile Publisher:Spandidos PublicationsJournal:Oncology Reports (issn: 1021-335X, eissn: 1791-2431,

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Authors: Torres, Cristian G.; Ramírez, María Eugenia; Cruz, Pamela; Epuñán, María José; Valladares Boasi, Luis; Sierralta, Walter D.;

doi: 10.3892/or.2011.1284

pmid: 21567091

handle: 10533/137547

27-Hydroxycholesterol induces the transition of MCF7 cells into a mesenchymal phenotype

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Abstract

A decrease in the expression of E-cadherin and β-catenin, paralleling the loss of adherens junction complex, was observed in MCF7 cells exposed for longer than 48 h to 2 µM 27-hydroxycholesterol (27OHC), indicating an epithelial-mesenchymal transition (EMT). Upon removal of 27OHC from the culture medium, the cells released by the exposure of 72 h to the oxysterol grew as loosely packed cell groups. In these cells, accumulation of E-cadherin and β-catenin in the cytoplasm and the prolonged expression of epidermal growth factor receptor 2 (EGFR2/neu) in the plasma membrane were observed, suggesting that the acquired phenotype was related to the expression of this tyrosine kinase-growth factor receptor. The results presented here are discussed on the basis of the claimed relationship between 27OHC, hypercholesterolemia, macrophage infiltration and therapy-resistant ERα+ breast cancer incidence.

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Chile

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Keywords

Human mammary cancer, Epithelial-Mesenchymal Transition, 27-hydroxycholesterol, MCF7 cells, Estradiol, E-cadherin, Breast Neoplasms, Adherens Junctions, Cell Growth Processes, β-catenin, Epidermal growth factor receptor 2/neu, Cadherins, Hydroxycholesterols, Adherens junction, Phenotype, Cell Line, Tumor, Animals, Humans, Female, beta Catenin

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