Detection of drug-resistance genes using single bronchoscopy biopsy specimens
Copyright policy )Detection of drug-resistance genes using single bronchoscopy biopsy specimens
Expression of three major resistance genes MDR1, MRP1 and LRP was investigated in small cell lung cancer, non-small cell lung cancer and metastasis. Single biopsies of bronchoscopy from 73 patients were performed to investigate expression of these three resistance genes by reverse transcriptase-polymerase chain reaction. Relations between gene expression and patient age, smoking status, histology, and chemotherapy were evaluated. A more frequent expression of MDR1 (77 versus 66%), MRP1 (91 versus 72%) and LRP (77 versus 63%) genes was detected in the malignant biopsies than in the non-malignant, respectively. In the metastasis biopsies, expression of these genes was markedly increased. No significant difference was observed between specimens before and after chemotherapy. Biopsies from progressing cancer showed higher MDR1, MRP1 and LRP gene expression. In conclusion, these data reveal a major role of MRP1 in intrinsic resistance and the high gene expression of MDR1 and MRP1 in relapsed diseases.
- Institut Jean Godinot France
- University of Reims Champagne-Ardenne France
Male, ATP-Binding Cassette, Sub-Family C Proteins, Lung Neoplasms, Biopsy, Middle Aged, Polymerase Chain Reaction, Radiation Tolerance, Drug Resistance, Multiple, Drug Resistance, Neoplasm, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, Bronchoscopy, Humans, Female, ATP Binding Cassette Transporter, Subfamily B, Member 1, Aged, DNA Primers
Male, ATP-Binding Cassette, Sub-Family C Proteins, Lung Neoplasms, Biopsy, Middle Aged, Polymerase Chain Reaction, Radiation Tolerance, Drug Resistance, Multiple, Drug Resistance, Neoplasm, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, Bronchoscopy, Humans, Female, ATP Binding Cassette Transporter, Subfamily B, Member 1, Aged, DNA Primers
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