Regulation of matrix metalloproteinase 13 expression by androgen in prostate cancer
Copyright policy )Regulation of matrix metalloproteinase 13 expression by androgen in prostate cancer
The matrix metalloproteinases (MMPs) are members of a family of endopeptidases that are able to degrade extra-cellular matrix. MMPs and their inhibitors, tissue inhibitors of metalloproteinases (TIMPs), play a key role in the migration of normal and malignant cell. Interaction of MMPs and TIMPs has been involved in the process of tumor invasion and metastasis. Using cDNA microarray as a screening tool to find androgen regulated gene in prostate cancer, we have found that expression of MMP-13 is regulated by androgen in prostate cancer derived cell line LNCaP. This regulation was further confirmed and quantified by real-time RT-PCR. In addition, the upregulation of MMP-13 mRNA by androgen could be abolished by the androgen antagonist Casodex but not the protein inhibitor cycloheximide. Western blot and immunohistochemistry of MMP-13 confirmed the androgen regulation at the protein level. We have furthermore shown that MMP-13 expression is presented in human prostate cancer obtained from aspiration biopsy. In summary, MMP-13 is androgen regulated and detectable in prostate cancer. Further study of MMP-13 in prostate cancer may help us to understand the progression of the cancer and can lead to new therapeutic options.
- Karolinska Institute Sweden
Male, Protein Synthesis Inhibitors, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Prostatic Neoplasms, Androgen Antagonists, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Immunoenzyme Techniques, Tosyl Compounds, Matrix Metalloproteinase 13, Nitriles, Androgens, Tumor Cells, Cultured, Humans, Anilides, Collagenases, RNA, Messenger, Cycloheximide, Oligonucleotide Array Sequence Analysis
Male, Protein Synthesis Inhibitors, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Prostatic Neoplasms, Androgen Antagonists, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Immunoenzyme Techniques, Tosyl Compounds, Matrix Metalloproteinase 13, Nitriles, Androgens, Tumor Cells, Cultured, Humans, Anilides, Collagenases, RNA, Messenger, Cycloheximide, Oligonucleotide Array Sequence Analysis
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