AIM: The study was conducted to evaluate the effect of combined low energy laser application and Micro-Osteoperforations versus the effect of the application of each technique separately on the rate of orthodontic tooth movement. PATIENTS AND METHODS: Three parallel groups (each group contained 10 patients) were performed; Group A: In which one side was controlled side, and the other side received micro-osteoperforations (MOPs), Group B: In which one side was controlled side, and the other side received low-level laser therapy (LLLT), Group C: In which one side was controlled side, and the other side received both MOPs and LLLT. RESULTS: Significant statistical differences were obvious in the rate of canine retraction between each intervention and the control sides as following; the MOPs increased the rate of canine retraction by 1.6 fold more than the control side, LLLT increased the rate of canine retraction by 1.3 fold than the control side, and combination of both techniques resulted in an increase in the rate of canine retraction by 1.8 fold more than the control side. CONCLUSION: Combination of MOPs and LLLT proved to be more efficient regarding increasing the rate of canine retraction than the application of each technique separately.