Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing
Copyright policy )Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing
Introduction: We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009. Methodology: This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing. Results: From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one such virus with H274Y mutation using pyrosequencing. Conclusions: The patient probably acquired oseltamivir resistance from natural variation, since he had never received that form of treatment before and recovered from influenza-like symptoms without using anti-influenza drugs.
- Mahidol University Thailand
- Ramathibodi Hospital Thailand
Adult, Male, Influenza A (H1N1), Epidemiology, Mutation, Missense, Biomedical Engineering, Structure and Function of Nicotinic Receptors, FOS: Medical engineering, Antiviral Agents, Microbiology, Gene, Disease Outbreaks, Breath Analysis Technology, Computational biology, Subtyping, Influenza A Virus, H1N1 Subtype, Oseltamivir, Engineering, Biochemistry, Genetics and Molecular Biology, Virology, Drug Resistance, Viral, Influenza, Human, Health Sciences, Genetics, Humans, Internal medicine, Molecular Biology, Biology, H1N1, Life Sciences, Pyrosequencing, Sequence Analysis, DNA, Thailand, RC31-1245, Computer science, QR1-502, Programming language, Amino Acid Substitution, Molecular Diagnostic Techniques, H274Y, FOS: Biological sciences, Physical Sciences, Medicine, Influenza Virus Research and Epidemiology
Adult, Male, Influenza A (H1N1), Epidemiology, Mutation, Missense, Biomedical Engineering, Structure and Function of Nicotinic Receptors, FOS: Medical engineering, Antiviral Agents, Microbiology, Gene, Disease Outbreaks, Breath Analysis Technology, Computational biology, Subtyping, Influenza A Virus, H1N1 Subtype, Oseltamivir, Engineering, Biochemistry, Genetics and Molecular Biology, Virology, Drug Resistance, Viral, Influenza, Human, Health Sciences, Genetics, Humans, Internal medicine, Molecular Biology, Biology, H1N1, Life Sciences, Pyrosequencing, Sequence Analysis, DNA, Thailand, RC31-1245, Computer science, QR1-502, Programming language, Amino Acid Substitution, Molecular Diagnostic Techniques, H274Y, FOS: Biological sciences, Physical Sciences, Medicine, Influenza Virus Research and Epidemiology
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