The work under new conditions of information technologies’ development and adoption of new forms of organizations, such as virtual organization, has not only advantages, but also causes certain problems such as personnel loyalty because of dispersion of personnel of virtual organizations. In the article the conception of loyalty and loyal employee, forms of organizational commitment, interaction of loyalty and motivation are analysed, the main factors of personnel loyalty formation in virtual organizations are identified. In the article is presented results and analysis of the research. The main task of the research was to identify the most important factors that determine loyalty of personnel and the main differences of these factors in virtual and traditional organizations.