Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
Copyright policy )Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
- The University of Texas System United States
- Texas A&M University United States
Macrophage Colony-Stimulating Factor, Macrophages, Cytological Techniques, Cell Polarity, Granulocyte-Macrophage Colony-Stimulating Factor, Bone Marrow Cells, Cell Differentiation, Macrophage Activation, Hematopoietic Stem Cells, Mice, Animals
Macrophage Colony-Stimulating Factor, Macrophages, Cytological Techniques, Cell Polarity, Granulocyte-Macrophage Colony-Stimulating Factor, Bone Marrow Cells, Cell Differentiation, Macrophage Activation, Hematopoietic Stem Cells, Mice, Animals
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