Introduction: Rooming-in is one of the baby-friendly hospital initiative steps. Rooming-in has positiveoutcomes for mothers and newborns. However, the effect of rooming-in on postpartum fatigue and thedifficulty in baby care activities is hardly investigated. Therefore, the purpose of this study is to identify andcompare difficulty in baby care activities and postpartum fatigue between rooming-in health care facilitiesand non- rooming-in facilities.Method: A Quasi- experimental design was used. A total of 152postpartum women were recruited from twohospitals in Jordan. Two self-administered questionnaires were used to collect data. Independent samplet-test was used to analyze the data.Results: The women in the rooming-in group scored significantly higher level of postpartum fatigue thanwomen in the non-rooming-in group. There were no significant differences between study groups related todifficulty in baby care activities.Conclusions: Postpartum rooming-in practice doesn`t affect the difficulty in baby care activities, but itinfluences the level of postpartum fatigue. Therefore, the health care providers should consider the level ofpostpartum fatigue among the women when implementing rooming-in.