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Journal of the Selva Andina Research Society
Article . 2016 . Peer-reviewed
Data sources: Crossref
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Journal of the Selva Andina Research Society
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License: CC BY NC
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Avaliação de protocolos para extração de DNA Genômico de sangue Bovino

Authors: do Amaral Crispim Bruno; André Vieira Nascimento; Ândrea Renata Romero da Silva; Juliana Fernandes dos Santos; Alexandre Campos Banari; Leonardo de Oliveira Seno; Alexéia Barufatti Grisolia;

Avaliação de protocolos para extração de DNA Genômico de sangue Bovino

Abstract

Estudos basicos sobre tecnicas de extracao de DNA sao de extrema importância para o sucesso de trabalhos cientificos no campo da biologia molecular. A extracao e a purificacao de acidos nucleicos constituem etapas fundamentais para o estabelecimento de posteriores analises geneticas. Objetivou-se neste trabalho avaliar a eficacia de diferentes protocolos de extracao de DNA por meio da determinacao de quantidade, qualidade e a possibilidade de amplificacao por meio de PCR do material genetico extraido. Utilizou-se amostras de sangue de dez animais, que foram submetidas a extracao de DNA e, logo apos a reacao em cadeia pela polimerase (PCR). Os resultados das analises obtidas por nanofotometria indicaram que a quantidade e a qualidade do DNA genomico foram consideradas satisfatorias, em todos os protocolos, para a realizacao da PCR, mas houve diferenca estatistica significativa entre os parâmetros analisados, tanto na quantidade quanto na qualidade (p < 0.05) do DNA obtido. O protocolo de extracao utilizando sangue total foi mais eficiente quanto ao tempo e a qualidade do DNA extraido, entretanto em todos os processos de extracao houve ausencia de degradacao. Tambem foi demonstrado a possibilidade de amplificacao da regiao do exon 2 do gene da leptina de bovinos no DNA extraido.

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    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
3
Average
Average
Average
gold