Antibodies(Abs) have been always a major place in diagnostic laboratories. Many diagnostic techniques like Enzyme-Linked Immuno Sorbent Assays (ELISA), immunofluorescence, Ab-microarray platforms, immunoblots, X-ray crystallography require the Abs. ELISA is the main test that used Abs for Foot and Mouth Disease (FMDV) serology. It needs polyclonal or monoclonal Abs to detect FMDV antigen or Abs. For this purpose, solid-phase competitive ELISA (SPCE) or liquid phase blocking ELISA (LPBE) and non-structural protein (NSP) ELISA are used. SPCE and LPBE have mainly used FMDV structural protein (SP)-Ab detection. In this study, it was aimed to produce a polyclonal Ab against FMDV ANep84 (Genotype VII) and OTUR07 (OPanAsia II), ATUR11 (A Iran05) strains for LPBE, FMDV SP-antibody detection. For this purpose, 4 guinea pigs and 6 rabbits were used for each serotype of FMDV. After producing Abs, checkerboard ELISA titration was performed to determine the optimal test dilution of Abs. Backgrounds, cross-reactions against three strains of FMDV were also checked. In conclusion, polyclonal Abs were produced against FMDV ANep84 (Genotype VII) and O Tur07 (O Pan Asia II), ATUR11 (A Iran05) strains, and standardized for LPBE test.