Oxidative stress has been known to play an important role in the pathogenesis of various neurodegenerative diseases. Dietary polyphenols and other natural antioxidants are the most popular compounds in clinical testing for the elimination of neurodegeneration. It has been demonstrated in recent studies that fruit of Zizyphus jujuba possesses several vital biological activities. This study intends to evaluate antioxidant activity of Zizyphus jujuba on human glioblastoma cells. Cell survival was quantified by colorimetric viability assay with dose response. Cells were pretreated with 100 μM Ziziphus jujuba essential oil for 1h, then 100 μM H2O2 was added to the cells for 12 hours. After that, the cell medium were taken after treatment period and replaced with fresh medium. Total oxidant capacity (TOS) and total antioxidant capacity (TAS) levels were estimated using specific colorimetric methods. Oxidative stress index (OSI) was calculated from the ratio of TOS and TAS values. Many researches have been reported that the essential oil from seeds helps to prevent the oxidative stress induced neuronal diseases. The antioxidant potential of Ziziphus jujuba may be attributed to the presence of flavonoids and the other constituents present therein. Our data suggested that Ziziphus jujuba is effective in preventing H2O2-induced oxidative stress.