The work is framed within Printed Electronics, an emerging technology for the manufacture of electronic products. Among the different printing methods, the roll-to-roll flexography technique is used because it allows continuous manufacturing and high productivity at low cost. Apart from the process parameters, the ink and the substrate properties are some of the variables associated with the flexographic printing. Specifically, this study investigates the ink penetration, the print uniformity, the adhesion, the fastness, and the electrical behaviour of the same conductive silver ink printed on different flexible substrates through the flexography process. In addition to polymeric and siliconized paper substrates, which are typical used in printed electronics, two substrates were also chosen for the study: woven and nonwoven fabric. Optical, scanning electronic microscope (SEM), 4-point Kelvin and colour fastness to wash and rubbing analyses have been performed. The results concluded that, regarding the conductivity behaviour, porous substrates like textiles and nonwoven fabrics without pre and post treatments do not present acceptable results, whereas polymers or silicone papers do. Nevertheless, woven and nonwoven fabrics are a suitable early option regarding colour fastness to wash instead of thin polymeric and paper substrates that tear at the wash machine. A solution for an optimal printing on textiles would be the surface substrates pre-treatment by applying different chemical compounds that increase the adhesion of the ink on the fabric