In this study, the identification of Vibrio isolates isolated from vibriosis infection of sea bass (Dicentrarchus labrax) and rainbow trout (Oncorhynchus mykiss) cultured in the Black Sea was carried out using conventional biochemical tests and PCR methods. Kidneys and spleens of moribund fish showing typical disease symptoms were inoculated onto tryptic soy agar (TSA) containing 1.5% salt and incubated at 20±2°C. Isolated colonies were purified by inoculation onto thiosulphate citrate bile salts sucrose agar (TCBS). The bacterial strains were found to be motile, Gr(-), catalase, and oxidase positive, sensitive to the O/129 test (150 µg), and fermentative in OF medium. According to PCR test results, all of isolated bacteria from sea bass were identified 19 isolates as V. anguillarum, 1 isolate as V. fluvialis, 7 isolates as V. harveyi, 19 isolates as V. parahemolyticus, and 1 isolate as V. vulnificus. All of bacteria isolated from rainbow trout were identified as two isolates V. alginolyticus and 29 isolates as V. anguillarum. In addition, according to the slide agglutination test results performed with Vibrio anguillarum O1 serotype, most of the isolates were determined to be Vibrio anguillarum. According to antimicrobial test results, florfenicol and enrofloxacin were identified as the most effective chemotherapeutic agents.