Introduction:This study aimed to investigate the phytochemical contents, antioxidant activities, and androgenic properties ofPeltophorum africanumSond,Trichilia emeticaVahl,Terminalia sambesiaca, andXimenia caffra.Methods:The finely powdered leaves of the selected plants were extracted using acetone, aqueous, and methanol as solvents. The total phenolics and flavonoids contents were determined from gallic acid and quercetin standard curves. The antioxidant activities of these extracts were evaluated using 1, 1-Diphenyl-2-picrylhydrazyl ( DPPH) assay. The effect of plant extracts (100-1000 µg/mL) on TM3 Leydig cells was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Testosterone levels were measured using ELISA kit.Results:The methanol extracts ofT. sambesiacaandX. caffrarevealed higher total phenolic and flavonoid contents (102.13±2.32 mg/g GAE and 1.05±0.04 mg/g QE, respectively) than other plant extracts. The acetone and methanol extracts ofP. africanumrevealed the best IC50value (12.50 ± 0.052 µg/mL) against DPPH than the other plant extracts and ascorbic acid. The MTT assay results showed that all varying concentrations of plant extracts maintained cell viability and were not cytotoxic with IC50values of greater than 20 µg/mL. The methanol extract ofT. sambesiacahad the highest testosterone production at 500 µg/mL (0.399 ng/mL) when compared with the basal control while at the concentration of 500 µg/mL the acetone extracts ofP. africanumandT. sambesiacahad significantly high testosterone production 0.147 and 0.188 ng/mL respectively when compared with basal control.Conclusion:The results reveal that these plants possess antioxidants and androgenic property and suggest the potential use for the treatment of male infertility.