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Nippon Saikingaku Zasshi
Article . 1990 . Peer-reviewed
Data sources: Crossref
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Nippon Saikingaku Zasshi
Article
Data sources: UnpayWall
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Nippon Saikingaku Zasshi
Article . 1991
Data sources: Europe PubMed Central
https://dx.doi.org/10.3412/jsb...
Article
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Claim

Spore-spheroplast transformation of Bacillus megaterium.

Ｂａｃｉｌｌｕｓ　ｍｅｇａｔｅｒｉｕｍの芽胞スフェロプラストを用いた形質転換
descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1990 Japanese Publisher:Japanese Society for BacteriologyJournal:Nippon Saikingaku Zasshi, volume 45, pages 841-843 (issn: 0021-4930, eissn: 1882-4110, Copyright policy )
Authors: Y, Takubo; J, Nishikawa; K, Tani; T, Nishihara; M, Kondo;

doi: 10.3412/jsb.45.841

pmid: 2125086

Spore-spheroplast transformation of Bacillus megaterium.

Abstract

A new method for transformation of Bacillus megaterium was developed by modification of Chang and Cohen's method. In our method, spore spheroplasts were used as recipient cells instead of the protoplasts of vegetative cells. Longer incubation (60 min) of spore spheroplasts and plasmid DNA before treatment with polyethylene glycol remarkably increased the efficiency of transformation. The frequency of transformation was about 10(4) per microgram of plasmid DNA. A shot-gun-type cloning of chromosome DNA of B. megaterium ATCC 12872 was available in B. megaterium ATCC 19213 strain by this transformation method.

Related Organizations
Keywords

DNA, Bacterial, Spores, Bacterial, Bacillus megaterium, Spheroplasts, Transformation, Bacterial, Cloning, Molecular, Plasmids

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
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