Background: Moist Heat Sterilization validation process comprises several steps. The IQ, OQ have to follow the specified SOPs of manufacturer or equipment supplier. However, the efficiency of the sterilization process must be expressed in numberized values based on sterilization coefficient D, Z value, F value and SAL value. Materials and methods: Studying to calculate the total Moist Heat Sterilization time based on the survey results process validation at 13 pharmaceutical factories having chains of sterile drugs and the other published documents. Results: The researched results were calculated the total sterilization time C (if the assumption that SAL = 10-6), this time will be compared with the real time of the process. Conclusion: The real time of the sterilization process is as similar as the C value, the efficiency of sterilization process is highlier. Keywords: Sterile drug, Moist Heat Sterilization, D value, Z value, F value, Biological indicator, process validation.