Laser-deposited gold immobilization layers with different porosities were incorporated into Love Wave Surface Acoustic Wave sensors (LW-SAWs). Acetylcholinesterase (AChE) enzyme was immobilized onto three gold interfaces with different morphologies, and the sensor response to chloroform was measured. The response of the sensors to various chloroform concentrations indicates that their sensing properties (sensitivity, limit of detection) are considerably improved when the gold layers are porous, in comparison to a conventional dense gold layer. The results obtained can be used to improve properties of SAW-based biosensors by controlling the nanostructure of the gold immobilization layer, in combination with other enzymes and proteins, since the design of the present sensor is the same as that for a Love Wave biosensor.