CO2 huff-n-puff is an important method for the development of shale oil reservoirs. In this study, HPMI and NMR technology was used to characterize the pore distribution of the cores. The CO2 huff-n-puff experiment experiments were conducted to study the effects of injection pressure, soaking time, and heterogeneity on the CO2 huff-n-puff. The results showed that the Jimsar core pores are predominantly nanopores. Mesopores with a pore radius between 2 nm and 50 nm accounted for more than 70%. CO2 huff-n-puff has been shown to effectively enhance shale oil recovery. When the injection pressure was greater than the miscible pressure, higher injection pressures were able to improve the recovery of macropores, particularly in cores with higher permeability. Appropriately extending the soaking time enhanced the diffusion of CO2 in the mesopores, and the recovery increased to above 10%. Determining the optimal soaking time is crucial to achieve maximum CO2 huff-n-puff recovery. Artificial fractures can enhance the recovery of mesopores around them, resulting in core recovery of up to 60%. However, artificial fractures exacerbate reservoir heterogeneity and reduce the CO2 huff-n-puff recovery of matrix. Increasing the cycles of CO2 huff-n-puff can effectively reduce the impact of heterogeneity on the recovery of matrix. In conclusion, expanding the area of the fracturing transformation zone and selecting the appropriate injection pressure and soaking time for the multiple cycles of CO2 huff-n-puff can effectively improve the recovery of shale oil reservoirs.