Increased demand for wood affects its price and thus contributes to the growing interest in raw materials that can be used as a partial or total substitute for wood in the production of particleboard. One of the raw materials for the production of particleboard can be Cannabis sativa or, more precisely, hemp shives. In this work, 7 variants of panels with a density of 650 kg/m3 with 10 and 25% hemp shives substitution in different layers were produced. Particleboards containing hemp shives were characterized by lower density compared to conventional particleboards. The shares of hemp shives at the levels of 10% and 25% have a slight impact on the MOR and MOE; additional IB showed no statistically significant differences between the conventional particleboards and particleboards with a share of hemp shives. For particleboards with 25% hemp shives, a reduction in swelling was observed relative to particleboards made entirely of industrial wood particles.