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Methods and Protocols
Article . 2018 . Peer-reviewed
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Methods and Protocols
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Methods and Protocols
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PubMed Central
Article . 2018
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https://dx.doi.org/10.3390/mps...
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Other literature type
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Improved Detection and Fragmentation of Disulphide-Linked Peptides

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 03 Sep 2018 English Publisher:MDPI AGJournal:Methods and Protocols, volume 1, page 33 (eissn: 2409-9279, Copyright policy )
Authors: Maes, Evelyne; Clerens, Stefan; Dyer, Jolon M.; Deb-Choudhury, Santanu;

doi: 10.3390/mps1030033

pmid: 31164574

pmc: PMC6481087

Improved Detection and Fragmentation of Disulphide-Linked Peptides

Abstract

Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-TOF-TOF MS). This improved assignment will be a significant new tool when a simple screening to confirm the DSB existence is required.

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  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 0
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Average
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green
gold