In this work, a compression experiment of 35CrMo steel is carried out over a wide range of temperatures (1123–1423 K) and strain rates (0.1–10 s−1) to obtain further understandings of the flow behaviors. The results show that the strain hardening effect of 35CrMo steel is stronger than that of dynamic recrystallization at low temperature and high strain rate; on the contrary, the rheological curves show typical dynamic recrystallization characteristics at high temperature and low strain rate. This indicates that the strain hardening and recrystallization behavior of 35CrMo steel is affected by temperature, strain and strain rate, and its true stress-strain curves can be observed typical work hardening and dynamic softening features. A modified Johnson-Cook (JC) model is developed to predict the flow stress of the alloy. The results of the comparison show that the predicted values of the modified JC model are in good agreement with the experimental values.