Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac
Copyright policy )Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac
CMP-Neu5Ac is an important sugar nucleotide for biosynthesis of sialic acid and its conjugates. In this paper, a large-scale production system of CMP-Neu5Ac by a single strain is reported. The co-expression of Neu5Ac aldolase (EC4.1.3.3) and CMP-Neu5Ac synthetase (EC 2.7.7.43) was achieved by constructing individual genes into one plasmid and having a single culture that has both NeuAc aldolase and CMP-Neu5Ac synthetase activities. Overall this system only employed N-acetylmannosamine, excess of pyruvate and CTP to produce CMP-Neu5Ac. This work has demonstrated that a large-scale synthesis of sialic acid-derived oligosaccharides could be achieved economically and efficiently through a single, biosynthetic pathway engineered microorganism.
- University of Minnesota System United States
- University of Minnesota United States
- State Key Laboratory of Microbial Technology China (People's Republic of)
- The Ohio State University United States
- Shandong Women’s University China (People's Republic of)
NeuAc aldolase, QH301-705.5, CMP-NeuAc synthetase, biosynthesis; cytidine 5’-monophospho-N-acetylneuraminic acid; NeuAc aldolase; CMP-NeuAc synthetase, cytidine 5’-monophospho-N-acetylneuraminic acid, biosynthesis, Biology (General)
NeuAc aldolase, QH301-705.5, CMP-NeuAc synthetase, biosynthesis; cytidine 5’-monophospho-N-acetylneuraminic acid; NeuAc aldolase; CMP-NeuAc synthetase, cytidine 5’-monophospho-N-acetylneuraminic acid, biosynthesis, Biology (General)
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