Italian honey bees (IHBs, Apis mellifera ligustica) exhibit superior comb-building abilities compared with Chinese honey bees (CHBs, Apis cerana cerana), which often fail to fully utilize wax foundations, resulting in incomplete comb structures. The present study aimed to accelerate comb construction in CHB colonies using IHBs. In the experiment, IHB colonies, each with approximately 42,000 adult workers, required over four hours to construct a semi-drawn comb on CHB wax foundations. These semi-drawn combs were then transferred to experimental CHB colonies, where they were left to complete the comb-building process for an additional 24 h (4 + 24 h relay). In contrast, control CHB colonies were allowed to build combs on fresh CHB wax foundations for 28 h. The results showed that the combs built by CHBs in 28 h, those built by IHBs in 4 h, and the 4 + 24 h relay combs all shared a foundation size of 41.7 cm × 19.7 cm, with average thicknesses of 10.40 mm, 5.60 mm, and 12.20 mm, respectively. The average percentage of cells built in the 4 + 24 h relay combs was significantly higher than that in the 28 h combs. Additionally, cells in the relay combs were significantly larger and deeper than those in the combs built solely by CHBs. Although these findings prove that utilizing IHB colonies to form a base structure can enhance the efficiency of comb construction in CHB colonies, further research is needed to confirm the effects of 4 + 24 h operation on brood rearing and worker bee size.