The present study is an update on the situation of potential vectors of Xylella fastidiosa in Tunisia. Investigations in nine Tunisian regions (Nabeul, Bizerte, Béja, Jendouba, Zaghouan, Kairouan, Ben Arous, Tunis and Manouba) from 2018 to 2021 allowed for the observation of 3758 Aphrophoridae among a total of 9702 Auchenorrhyncha individuals collected by sweep net. Four Aphrophoridae species were identified with Philaenus tesselatus as most abundant (62%), followed by Neophilaenus campestris (28%), Neophilaenus lineatus (5%) and Philaenus maghresignus (5%). Aphrophoridae individuals were found to be particularly abundant in both forests of Nabeul and Jendouba, secondarily in olive groves and dry grassland. Furthermore, their distribution on weed hosts was followed in these two regions where nymphs and adults are widely distributed. P. tesselatus appears to be the most abundant species as determined either by conventional sweep netting for adults or by plant sampling on Sonchus, Smyrnium, Cirsium, Rumex, Polygonum and Picris for nymphs. Limited numbers of adults of P. maghresignus were detected by sweep netting, while nymphs of this species were found on Asphodelus microcarpus only. N. campestris was found in high numbers on plants belonging to the Poaceae family in forests, dry grassland and olive groves whereas N. lineatus occurred on herbs under or near olive trees and in dry grasslands.