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Localization of MLH3 at the Centrosomes

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 11 Aug 2014 Germany Publisher:MDPI AGJournal:International Journal of Molecular Sciences, volume 15, pages 13,932-13,937 (eissn: 1422-0067,

Authors: Roesner, Lennart M; Mielke, Christian; Faehnrich, Silke; Merkhoffer, Yvonne; Dittmar, Kurt E J; Drexler, Hans G; Dirks, Wilhelm G;

doi: 10.3390/ijms150813932

pmid: 25116689

pmc: PMC4159832

handle: 10033/332725

Localization of MLH3 at the Centrosomes

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Abstract

Mutations in human DNA mismatch repair (MMR) genes are commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 protein heterodimerizes with PMS2, PMS1, and MLH3 to form MutLα, MutLβ, and MutLγ, respectively. We reported recently stable expression of GFP-linked MLH3 in human cell lines. Monitoring these cell lines during the cell cycle using live cell imaging combined with confocal microscopy, we detected accumulation of MLH3 at the centrosomes. Fluorescence recovery after photobleaching (FRAP) revealed high mobility and fast exchange rates at the centrosomes as it has been reported for other DNA repair proteins. MLH3 may have a role in combination with other repair proteins in the control of centrosome numbers.

Country

Germany

Related Organizations

Leibniz Association
Germany
Helmholtz Association of German Research Centres
Germany
Hannover Medical School
Germany
Heinrich Heine University Düsseldorf
Germany
Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures
Germany

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Keywords

Centrosome, HEK293 Cells, Microscopy, Confocal, MutL Proteins, Communication, Humans, Carrier Proteins, Fluorescence Recovery After Photobleaching

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