Soil erosion is the displacement of soil’s upper layer(s) triggered by a variation in topography, land use and soil types, and anthropogenic activities. This study selected the Marrowbone Creek-Russel Fork watershed in eastern Kentucky to estimate the mean annual soil loss over eight years (from 2013 to 2020) utilizing the Universal Soil Loss Equation (USLE). We included monthly precipitation, soil survey, digital elevation model (DEM), and land cover data to estimate the parameters of the USLE. The mean annual soil loss for the study area ranged from 1.77 to 2.91 Mg ha−1 yr−1 with an eight-year mean of 2.31 Mg ha−1 yr−1. In addition, we observed that developed land cover classes were less erosion-resistant than undeveloped land cover classes over the observation period. The results of this case study in our small watershed that has been historically impacted by upstream coal-mining activities are comparable to the results from similar studies in other geographic regions. However, we suggest other researchers conduct similar studies using robust data to determine the applicability of the USLE model and validate the results in developing measures to address soil loss issues.