In order to get rid of the dependence of the navigation and positioning system on the global navigation satellite system (GNSS), radio, television, satellite, and other signals of opportunity (SOPs) can be used to achieve receiver positioning. The space-based SOPs based on satellites offer better coverage and availability than ground-based SOPs. Based on the related research of Iridium SOPs positioning in the open environment, this paper mainly focuses on the occluded environment and studies the Iridium SOPs positioning technique in weak signal environment. A new quadratic square accumulating instantaneous Doppler estimation algorithm (QSA-IDE) is proposed after analysing the orbit and signal characteristics of the Iridium satellite. The new method can improve the ability of the Iridium weak signal Doppler estimation. The theoretical analysis and positioning results based on real signal data show that the positioning based on Iridium SOPs can be realized in a weak signal environment. The research broadens the applicable environment of the Iridium SOPs positioning, thereby improving the availability and continuity of its positioning.