Background: Automation of production processes is not just a simple replacement of a person in production, but it should lead to the success of an organization and contribute to the sustainable development of society and the natural environment. The aim of our study was to find out whether the level of automation of production processes affects the proportion of night work hours of production workers and whether employers are willing to automate production processes to achieve a lower number of night work hours. Methods: We used a quantitative approach to collect primary data through the survey method. The questionnaire was completed by 502 large and medium-sized manufacturing companies in Slovenia. Results: We found no statistically significant correlation between the level of automation of production processes and the percentage of night work hours of production workers. We also found that the reduction of the proportion of night work does not appear to be the main motivator for the introduction of automation of production processes. Conclusions: Based on the results, we rejected the assumption that automation of production processes has a direct impact on the proportion of night work. Moreover, our study will benefit all those who are concerned with the automation of production processes and night work.