Electronic money (e-money or e-Cash) is the digital representation of physical banknotes augmented by added use cases of online and remote payments. This paper presents a novel, anonymous e-money transaction protocol, built based on physical unclonable functions (PUFs), titled PUF-Cash. PUF-Cash preserves user anonymity while enabling both offline and online transaction capability. The PUF’s privacy-preserving property is leveraged to create blinded tokens for transaction anonymity while its hardware-based challenge–response pair authentication scheme provides a secure solution that is impervious to typical protocol attacks. The scheme is inspired from Chaum’s Digicash work in the 1980s and subsequent improvements. Unlike Chaum’s scheme, which relies on Rivest, Shamir and Adlemans’s (RSA’s) multiplicative homomorphic property to provide anonymity, the anonymity scheme proposed in this paper leverages the random and unique statistical properties of synthesized integrated circuits. PUF-Cash is implemented and demonstrated using a set of Xilinx Zynq Field Programmable Gate Arrays (FPGAs). Experimental results suggest that the hardware footprint of the solution is small, and the transaction rate is suitable for large-scale applications. An in-depth security analysis suggests that the solution possesses excellent statistical qualities in the generated authentication and encryption keys, and it is robust against a variety of attack vectors including model-building, impersonation, and side-channel variants.