The real-time monitoring of plant water status is an important issue for digital irrigation to increase water productivity. This work focused on a comparison of three biosensors that continuously evaluate plant water status: trunk microtensiometers (MTs), trunk time-domain reflectometry (TDR), and LVDT sensors. During the summer and autumn seasons (DOY 150–300), nectarine trees were subjected to four different consecutive irrigation periods based on the soil Management Allowed Deficit (MAD) concept, namely: MAD10 (light deficit); MAD50 (moderate deficit); MAD100 (severe deficit), and MAD0 (full irrigation). Measurements of stem water potential (Ψstem) and leaf gas exchange were recorded on representative days. A continuous measurement of the plant water status of Ψtrunk, MDS, and Ktrunk revealed the water deficits imposed on the soil. The highest water deficit observed at the end of the MAD100 period (Ψstem = −2.04 MPa and Ɵv = 17%) resulted in a minimum value of Ψtrunk (−1.81 MPa). The maximum value of MDS (408 µm) was observed earlier than that of Ψtrunk, motivated by the low sensitivity of MDS at Ψtrunk < −1.2 MPa and Ψstem < −1.5 MPa due to a decrease in the tissue elasticity of the trunk when severe water deficit conditions are reached. Both Ψtrunk and Ψstem were more dependent on soil water content, while MDS was more responsive to environmental changes. Ktrunk was the weakest indicator for determining plant water status, although when expressed as a daily fraction of depletion (KtrunkFD), it improved, evidencing a process of hysteresis. Ψtrunk showed the highest sensitivity, suggesting the potential use of MTs as a valuable biosensor for monitoring nectarine water status in digital agrosystems.