A Redox-Neutral, Two-Enzyme Cascade for the Production of Malate and Gluconate from Pyruvate and Glucose

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 26 May 2021 United Kingdom English Publisher:MDPI AGJournal:Applied Sciences, volume 11, page 4,877 (eissn: 2076-3417,

Copyright policy )Funded by:UKRI | Cascade processes for int..., UKRI | A tandem bio-chemo cataly...

Authors: Ravneet Mandair; Pinar Karagoz; Roslyn M. Bill;

doi: 10.3390/app11114877

A Redox-Neutral, Two-Enzyme Cascade for the Production of Malate and Gluconate from Pyruvate and Glucose

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Abstract

A triple mutant of NADP(H)-dependent malate dehydrogenase from thermotolerant Thermococcus kodakarensis has an altered cofactor preference for NAD+, as well as improved malate production compared to wildtype malate dehydrogenase. By combining mutant malate dehydrogenase with glucose dehydrogenase from Sulfolobus solfataricus and NAD+/NADH in a closed reaction environment, gluconate and malate could be produced from pyruvate and glucose. After 3 h, the yield of malate was 15.96 mM. These data demonstrate the feasibility of a closed system capable of cofactor regeneration in the production of platform chemicals.

Country

United Kingdom

Related Organizations

Aston University
United Kingdom
University College London
United Kingdom

Keywords

glucose dehydrogenase, Technology, QH301-705.5, T, Physics, QC1-999, malate dehydrogenase, enzymatic cascade reaction, Engineering (General). Civil engineering (General), Chemistry, TA1-2040, Biology (General), malate production, QD1-999

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