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Mass Spectrometry for Characterizing Plant Cell Wall Polysaccharides

قياس الطيف الكتلي لتوصيف السكريات الحائطية للخلية النباتية
Authors: Štefan Bauer;

Mass Spectrometry for Characterizing Plant Cell Wall Polysaccharides

Abstract

La spectrométrie de masse est une technique sélective et puissante pour obtenir des informations d'identification et de structure sur les composés présents dans les mélanges complexes. Comme elle ne nécessite qu'une petite quantité d'échantillon, c'est un excellent outil pour les chercheurs intéressés à détecter les changements dans la composition des glucides complexes des plantes. Cette mini-revue donne un aperçu des techniques courantes de spectrométrie de masse appliquées à l'analyse des glucides de la paroi cellulaire des plantes. Elle présente des exemples dans lesquels la spectrométrie de masse a été utilisée pour élucider la structure des oligosaccharides dérivés des hémicelluloses et des pectines et illustre comment les informations sur la séquence, les liaisons, la ramification et les modifications sont obtenues à partir des modèles de fragmentation caractéristiques.

La espectrometría de masas es una técnica selectiva y poderosa para obtener identificación e información estructural sobre compuestos presentes en mezclas complejas. Dado que solo requiere una pequeña cantidad de muestra, es una excelente herramienta para los investigadores interesados en detectar cambios en la composición de carbohidratos complejos de las plantas. Esta mini revisión brinda una descripción general de las técnicas comunes de espectrometría de masas aplicadas al análisis de carbohidratos de la pared celular de las plantas. Presenta ejemplos en los que la espectrometría de masas se ha utilizado para dilucidar la estructura de los oligosacáridos derivados de hemicelulosas y pectinas e ilustra cómo se obtiene información sobre secuencia, enlaces, ramificación y modificaciones a partir de patrones de fragmentación característicos.

Mass spectrometry is a selective and powerful technique to obtain identification and structural information on compounds present in complex mixtures.Since it requires only small sample amount it is an excellent tool for researchers interested in detecting changes in composition of complex carbohydrates of plants.This mini-review gives an overview of common mass spectrometry techniques applied to the analysis of plant cell wall carbohydrates.It presents examples in which mass spectrometry has been used to elucidate the structure of oligosaccharides derived from hemicelluloses and pectins and illustrates how information on sequence, linkages, branching, and modifications are obtained from characteristic fragmentation patterns.

قياس الطيف الكتلي هو تقنية انتقائية وقوية للحصول على معلومات تعريفية وبنيوية حول المركبات الموجودة في المخاليط المعقدة. نظرًا لأنها تتطلب كمية عينة صغيرة فقط، فهي أداة ممتازة للباحثين المهتمين باكتشاف التغيرات في تكوين الكربوهيدرات المعقدة للنباتات. تقدم هذه المراجعة المصغرة نظرة عامة على تقنيات قياس الطيف الكتلي الشائعة المطبقة على تحليل كربوهيدرات جدار الخلية النباتية. يقدم أمثلة على استخدام قياس الطيف الكتلي لتوضيح بنية السكريات القليلة المشتقة من نصف الخلية والبكتين ويوضح كيفية الحصول على المعلومات حول التسلسل والروابط والتفرع والتعديلات من أنماط التجزئة المميزة.

Keywords

carbohydrates, Carbohydrates, Oligosaccharides, Plant Science, Biochemistry, Mass Spectrometry, SB1-1110, Agricultural and Biological Sciences, oligosaccharides, Cell Wall, Biochemistry, Genetics and Molecular Biology, Polysaccharide, Molecular Biology, Biology, mass spectrometry, Chromatography, Molecular Structure, Mass spectrometry, Cell wall, Botany, Plant culture, Life Sciences, Plant Nutrient Uptake and Signaling Pathways, Chemistry, Molecular Mechanisms of Pollen Development and Function, cell wall, Structure and Function of Plant Cell Walls

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    popularity
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    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
48
Top 10%
Top 10%
Top 10%
Green
gold