Experimental method was employed to investigate mechanical characterization of carbon fiber reinforcement polymer (CFRP) composite beams at different temperatures. The experimental research is mainly based on the vibration response measurement by using the technique of electronic speckle pattern interferometry (ESPI), which can provide visualization of vibrating information represented as fringe patterns. The first ten order of out-of-plane mode shapes and natural frequencies at different temperatures are obtained by ESPI method. Based on the Euler-Bernoulli beam theory, the flexural moduli of the CFRP laminate was determined. The changes in the vibration response and modes of 0 degree off-axis CFRP beams at different temperatures has been investigated in this paper.