To the Editor: Dermacentor spp. ticks are found in many countries in Europe, and are vectors for several pathogens, including Francisella tularensis, Coxiella burnetii, Rickettsia spp., and Babesia canis (1,2). Because Dermacentor marginatus ticks require warm dry habitats, these ticks are found in only a few areas in southern Germany, mainly in the Rhine and Main valleys (3). However, these ticks may spread northwards because of increasing temperatures. In contrast, D. reticulatus ticks are present throughout Germany. Fourteen Rickettsia spp. are currently identified as human pathogens. The severity of human diseases differs among these species, ranging from mild to lethal illness (4). On the basis of serologic and genotypical characteristics, rickettsiae are divided into typhus and spotted fever groups. Within each group, antigenic differences are small, resulting in cross-reactivity that complicates differentiation of Rickettsia spp. by serologic methods. Therefore, PCR of eschar biopsy samples is a useful tool for diagnosis of rickettsial diseases (5). Little information exists regarding the prevalence of Rickettsia spp. in D. marginatus and D. reticulatus ticks in Germany. R. raoultii was recently detected in 23% of D. reticulatus ticks (6). In 1977, Rehacek et al. identified R. slovaca in 12% of Dermacentor spp. ticks from southern Baden-Wuerttemberg (7). Since then, this pathogen has not been detected in Germany. R. slovaca, a member of the spotted fever group, causes tick-borne lymphadenopathy, a relatively mild rickettsiosis (8). We report detection of R. slovaca in 5 of 666 Dermacentor spp. ticks from southern Germany. Moreover, we identified a case of tick-borne lymphadenopathy from Rhineland-Palatinate. We collected 666 adult Dermacentor spp. ticks by blanket-dragging; 26 were collected along the Main River near Aschaffenburg (Bavaria), and 640 from the Rhine Valley near Lorrach (Baden-Wuerttemberg). Ticks were homogenized, and the DNA was isolated by using the Maxwell 16 Instrument (Promega, Madison, WI, USA). For detection of rickettsiae, a TaqMan real-time PCR with the LightCycler system (Roche Diagnostics, Mannheim, Germany) was performed according to Wolfel et al. (9). A primer pair amplified a 70-bp fragment of the citrate synthase (gltA) gene. All positive samples were also tested with a PCR specific for the outer membrane protein A (rOmpA) gene (10). This amplification yielded an rOmpA fragment of 532 bp. Amplified products were analyzed by agarose gel electrophoresis. For identification of Rickettsia spp., amplification products of the rOmpA PCR were sequenced by using fluorescence-labeled dideoxynucleotide technology (Applied Biosystems, Darmstadt, Germany). Separation of sequenced fragments and data collection were performed by using an ABI PRISM 310 Genetic Analyzer (Applied Biosystems). All obtained sequences were analyzed and compared by using BLAST (www.ncbi.nlm.nih.gov/BLAST). Rickettsia spp. was detected by both PCR methods in 31% of 666 Dermacentor spp. ticks examined. Sequencing of part of the rOmpA gene showed that sequences of 5 samples (0.75%) from Aschaffenburg were R. slovaca, showing 100% similarity with a sequence deposited in GenBank (accession no. {"type":"entrez-nucleotide","attrs":{"text":"U43808","term_id":"1174142","term_text":"U43808"}}U43808). Only D. marginatus ticks from Aschaffenburg were infected with R. slovaca, and 202 ticks from Lorrach were infected with R. raoultii. We also identified a case of R. slovaca infection in southern Rhineland-Palatinate. The patient reported a tick bite; the tick was identified as Dermacentor spp. Fever, lymphadenopathy of submandibular lymph nodes, and exanthema at the site of the tick bite developed 7 days later. Serologic examinations by using an immunofluorescent test (Focus Diagnostics, Cypress, CA, USA) showed antibody titers of 64 for immunoglobulin (Ig) M and 1,024 for IgG against rickettsiae of the spotted fever group. These results indicated an acute rickettsial infection. Because of strong cross-reactivity among all species in the spotted fever group, we cannot differentiate between antibodies against R. slovaca and other species in this group. However, another immunofluorescent test for typhus group rickettsiae showed negative results, confirming that the patient was infected with spotted fever group rickettsiae. Results of PCRs specific for gltA and rOmpA of the patient’s tick were positive. We identified R. slovaca by sequencing the rOmpA gene. The sequence obtained showed 100% similarity with sequences in ticks from Aschaffenburg. Clinical symptoms, serologic results, and detection of R. slovaca in the tick from the patient strongly indicate that the patient had tick-borne lymphadenopathy caused by R. slovaca. The high prevalence of R. raoultii in Dermacentor spp. ticks is of concern because this species can also cause tick-borne lymphadenopathy, although R. raoultii is less pathogenic than R. slovaca (8). Tick-borne lymphadenopathy should be considered in the differential diagnosis of tick-borne diseases. The extent of the distribution of R. slovaca and R. raoultii in Germany remains to be elucidated.