Influenza A H5N1 Detection
Copyright policy )Influenza A H5N1 Detection
Nous avons développé un test sensible et rapide de transcription inverse en temps réel par réaction en chaîne de la polymérase (RT-PCR) pour détecter le virus de la grippe A H5N1 dans des échantillons cliniques. Ce test a été évalué avec des échantillons de patients infectés par le H5N1 et a démontré une plus grande sensibilité et un délai d'exécution plus rapide que la RT-PCR imbriquée.
Desarrollamos un ensayo sensible y rápido de reacción en cadena de la polimerasa con transcripción inversa en tiempo real (RT-PCR) para detectar el virus de la influenza A H5N1 en muestras clínicas. Este ensayo se evaluó con muestras de pacientes infectados con H5N1 y demostró una mayor sensibilidad y un tiempo de respuesta más rápido que la RT-PCR anidada.
We developed a sensitive and rapid real-time reverse transcription-polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinical samples. This assay was evaluated with samples from H5N1-infected patients and demonstrated greater sensitivity and faster turnaround time than nested RT-PCR.
طورنا اختبارًا حساسًا وسريعًا في الوقت الفعلي للتفاعل المتسلسل للبوليميراز والنسخ العكسي (RT - PCR) للكشف عن فيروس الأنفلونزا A H5N1 في العينات السريرية. تم تقييم هذا الفحص باستخدام عينات من مرضى مصابين بفيروس H5N1 وأظهر حساسية أكبر ووقت دوران أسرع من RT - PCR المتداخلة.
- National Institute Of Hygiene And Epidemiology Viet Nam
- Department of Health China (People's Republic of)
Time Factors, Epidemiology, LightCycler, Influenza A virus subtype H5N1, Keywords: human influenza A H5N1 virus, Infectious and parasitic diseases, RC109-216, Sensitivity and Specificity, Microbiology, Gene, Real-time polymerase chain reaction, nested RT-PCR, Virology, Influenza, Human, Health Sciences, Epidemiology and Pathogenesis of Respiratory Viral Infections, Reverse transcriptase, Genetics, Humans, Antigens, Viral, Biology, Management and Epidemiology of Pneumonia, Influenza A Virus, H5N1 Subtype, clinical samples, Reverse Transcriptase Polymerase Chain Reaction, Messenger RNA, H1N1, R, Dispatch, Turnaround time, Computer science, Polymerase chain reaction, Virus, molecular detection, Operating system, Hemagglutinins, Influenza A virus, FOS: Biological sciences, Reverse transcription polymerase chain reaction, Nested polymerase chain reaction, RNA, Viral, Medicine, Host Immune Response, real-time PCR, Influenza Virus Research and Epidemiology
Time Factors, Epidemiology, LightCycler, Influenza A virus subtype H5N1, Keywords: human influenza A H5N1 virus, Infectious and parasitic diseases, RC109-216, Sensitivity and Specificity, Microbiology, Gene, Real-time polymerase chain reaction, nested RT-PCR, Virology, Influenza, Human, Health Sciences, Epidemiology and Pathogenesis of Respiratory Viral Infections, Reverse transcriptase, Genetics, Humans, Antigens, Viral, Biology, Management and Epidemiology of Pneumonia, Influenza A Virus, H5N1 Subtype, clinical samples, Reverse Transcriptase Polymerase Chain Reaction, Messenger RNA, H1N1, R, Dispatch, Turnaround time, Computer science, Polymerase chain reaction, Virus, molecular detection, Operating system, Hemagglutinins, Influenza A virus, FOS: Biological sciences, Reverse transcription polymerase chain reaction, Nested polymerase chain reaction, RNA, Viral, Medicine, Host Immune Response, real-time PCR, Influenza Virus Research and Epidemiology
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