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Experimental Biology and Medicine
Article . 1949 . Peer-reviewed
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Low Speed Microtomy for the Electron Microscope

Authors: R P, RHOADES;

Low Speed Microtomy for the Electron Microscope

Abstract

The beam of a 50-kv electron microscope does not penetrate specimens that are thicker than 0.1 μ. Thus, in order to study biological specimens with this type of microscope we have further developed the technic described by Pease and Baker,1 who altered a Spencer Rotary Microtome (model 820) by adding a wedge to the mechanism for forward movement. We reduced the angle of the inclined plane surface by a factor of 10 to 1 so that each step is 0.1 μ rather than 1.0 μ.Since forward movement results from a pin in sliding contact with an inclined plane surface, it is important that the surface of the plane be as nearly perfectly flat as possible. The accompanying photograph, Fig. 1, illustrates the means by which the problem of a flat surface for even forward movement was solved. At the left of the picture (A) the original plane can be seen. The reduced angle is shown by D. The light gray triangular area (B) is an angle-reducing block of steel, to which is fastened an optical flat (C). A brass disc (E) instead o...

Keywords

Microscopy, Microscopy, Electron, Tissues, Electrons, Microtomy

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
2
Average
Top 10%
Average
gold