
pmid: 18139841
The beam of a 50-kv electron microscope does not penetrate specimens that are thicker than 0.1 μ. Thus, in order to study biological specimens with this type of microscope we have further developed the technic described by Pease and Baker,1 who altered a Spencer Rotary Microtome (model 820) by adding a wedge to the mechanism for forward movement. We reduced the angle of the inclined plane surface by a factor of 10 to 1 so that each step is 0.1 μ rather than 1.0 μ.Since forward movement results from a pin in sliding contact with an inclined plane surface, it is important that the surface of the plane be as nearly perfectly flat as possible. The accompanying photograph, Fig. 1, illustrates the means by which the problem of a flat surface for even forward movement was solved. At the left of the picture (A) the original plane can be seen. The reduced angle is shown by D. The light gray triangular area (B) is an angle-reducing block of steel, to which is fastened an optical flat (C). A brass disc (E) instead o...
Microscopy, Microscopy, Electron, Tissues, Electrons, Microtomy
Microscopy, Microscopy, Electron, Tissues, Electrons, Microtomy
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