
pmid: 1257262
A plasma culture system has been used successfully to grow and quantitate megakaryocyte colonies from mouse bone marrow following their staining for acetylcholinesterase activity in situ. Colonies averaging about six acetylcholinesterase-positive cells appear with a peak incidence after 4 days in culture with a plating efficiency of one colony formed for every 10(4) nucleated cells plated.
Mice, Acetylcholinesterase, Animals, Bone Marrow Cells, Cell Differentiation, Female, Mice, Inbred Strains, Megakaryocytes
Mice, Acetylcholinesterase, Animals, Bone Marrow Cells, Cell Differentiation, Female, Mice, Inbred Strains, Megakaryocytes
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