descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1971 English Publisher:Frontiers Media SAJournal:Experimental Biology and Medicine, volume 138, pages 213-215 (issn: 1535-3702, eissn: 1535-3699,

Authors: Garcia, J F; Schooley, J C;

doi: 10.3181/00379727-138-35865

pmid: 4942187

Dissociation of Erythropoietin from Erythropoietin-Antierythropoietin Complex

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Abstract

SummaryThe biological activity of human urinary erythropoietin is completely neutralized when combined with serum from rabbits immunized with erythropoietin. Acidification to pH 5.0, followed by heating in a boiling water bath for 5 min, recovers essentially all the erythropoietic biological activity from such a combination. These data further support the immunologic nature of the reaction between erythropoietin and the neutralizing serum.

Related Organizations

University of California, San Francisco
United States
University of California, Berkeley
United States

Keywords

Hot Temperature, Freund's Adjuvant, Radioimmunoassay, Antigen-Antibody Complex, Iron Isotopes, Antibodies, Hematocrit, Immunologic Techniques, Animals, Humans, Biological Assay, Immunization, Citrates, Rabbits, Acids, Erythropoietin

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