Calendula (Calendula officinalis) has healing and antiseptic properties with actions that are sudorific and analgesic, affect the bile duct, are anti-inflammatory, antiviral and anti-emetic, and tone the skin with vasodilatation. A tincture of 5% calendula positively influences the generation of new cells involved in wound healing, the fibroblasts, and provides more satisfactory healing than other treatments applied to wounds. Calendula officinalis plant extracts show anti-cancerous activity on various tumor cell lines derived from leukemias, fibrosa comas, melanomas, breast, cervix, prostate, pancreas and lung. It has also been internally used for the treatment of gastritis, colitis and bleeding of duodenal ulcers. The aqueous/ethanolic extract of Calendula flowers caused relaxation of spontaneous contraction and K+ induced contraction of muscles. When the extract was further fractionated with dichloromethane, it inhibited spontaneous contraction. Calcium channel blockade (CCB) was responsible for spasmolytic activity. N-type calcium channel blockade prevents sudden cardiac death. N-type calcium channel blockade (NCC) blockade only or along with L-type calcium channel blockade (LCC) blockade can be beneficial in patients with hypertension, cardiovascular and other metabolic diseases. Hepatoprotective Activity Calendula flower hydro-alcoholic extract caused 28.5% reduction in hepatocytolysis of CCl4 –intoxicated rat liver due to reduction in glutamo-pyruvate-transaminase and glutamo-oxalate-transaminase. Calendula flower hot water extract showed anti-hepatoma activity (25-26% inhibition) against five human liver cancer cells: Hep3B, SK-HEP-1, HepG2/C3A, PLC/ PRF/5 and HA22T/VGH. Moreover, CCl4 intoxicated rats pre-treated with Calendula floral extract afford a protection against CCl4 induced toxicity and showed an improvement in liver function due to significant anti-oxidant activity and free radical scavenging activity of bioactive metabolites including flavonoids and terpenoids present in Calendula. These bioactive metabolites have potent activities for scavenging the hydroxyl radicals (OH) and superoxide radicals (O.2) resulted from CCl4 metabolites. The aqueous-ethanolic extract of Calendula flowers exhibit a genotoxic effect at high concentration and anti-genotoxic effect at low concentration. Calendula glycosides show anti-inflammatory activities against mouse ear edema and calenduloside F 6’-O-n-butyl ester show potent cytotoxicity against melanoma, leukemia and colon cancer. C. officinalis inflorescence extract shows anti-inflammatory activity against the dextran and carrageenan-induced acute paw edema in mice. Abscesses, acne, amenorrhea, analgesia, anemia, antibacterial, antifungal, anti-inflammatory, antioxidant, anti-viral, anxiety, appetite stimulant, atherosclerosis, athlete’s foot, bacterial infections, benign prostatic hypertrophy, bladder irritation, blood purification, blood clots, bowel irritation, bruises, burns, cardiac disease, cholera, circulation, colitis, conjunctivitis, constipation, cosmetic, cough, cramps, diaper rash, dizziness, diuresis, dystrophic nervous disturbances, eczema, edema, epididymitis, epistaxis, eye inflammation, fatigue, fever, frostbite, gastrointestinal tract disorders, gastritis, gingivitis, gout, headache, heart disease, hemorrhoids, herpes simplex, herpes keratitis, HIV, indigestion, immunostimulant, influenza, insomnia, jaundice, liver cancer, liver-gallbladder function stimulator, menstrual period abnormalities, metabolic disorders, mouth and throat infections, muscular atrophy, nausea, nosebleed, pain, peptic ulcer disease, periodontal prophylaxis proctitis, prostatitis, purging agent, skin cancer, sore throat, spasms, spleen disorders, stomach ulcers, stones, syphilis, thrombophlebitis, tinnitus, toothache, tuberculosis, ulcerative colitis, urinary retention, uterine tonic, varicose ulcers, warts, yeast infections. Calendula officinalis extract was found to scavenge superoxide radicals generated by photo reduction of riboflavin and hydroxyl radicals generated by Fenton reaction and inhibited in vitro lipid peroxidation.