
doi: 10.31466/kfbd.353777
Spectrophotometric DPPH method was used for the determination of DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity of Scenedesmus sp. which was isolated from the Giresun mine and cultivated under BG11 culture conditions. 1 mL of 10-3 M DPPH ethanol solution was added and 3 mL of extract solution was added and vortexed. The absorbance was read at 517 nm for 30 minutes in the dark. BHT and Trolox were used as standard antioxidants. The DPPH radical scavenging activity reaction was calculated using the formula.
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