Introduction: Open fractures are fairly common in developing countries. Infection is a common complication of open fractures. Pre-debridement, post-debridement, and surgical site infection (SSI) cultures are important to identify microbiological profiles to prevent infection. Methods: We report microbiological profile of pre-debridement, post-debridement, and surgical site infection on open fracture in orthopedic patients at Soebandi General Hospital. 30 patients were taken into the study of all ages. Primarily, wound was examined and a description of the wound was recorded at 1st culture swab taken at the time of examination before debriment, followed by 2nd culture swab on 1st dressing after debridement, and 3rd culture swab if an infection continued further. Culture reports were collected for studying the pattern of bacterial isolate. Results: In this study, 30 patients became the study samples. The results of pre-debridement from 15 patients who had positive culture, 9 patients (56.25%) showed growth of Klebsiella sp., 3 patients (18.75%) showed growth of Proteus sp., 2 patients (12.50%) showed growth of Salmonella thypi, 1 patient (6.25%) showed growth of Shigella sonnei, and 1 patient (6.25%) showed growth of Staphylococcus aureus, and one patient culture grew several organisms. In post-debridement culture in the operating room, the results of culture identification from 12 patients showed that 5 patients (41.67%) had Klebsiella sp., 3 patients (25.00%) had Shigella sonnei, 2 patients (16.67) had Pseudomonas aeruginosa, 1 patient (8.33%) had Salmonella thypi, 1 patient (8.33%) had Proteus sp. Nine patients (30%) developed surgical wound infection with 6 patients (85.71%) showed growth of Staphylococcus aureus, 1 patient (14.29%) showed growth of Salmonella thypi, and 2 others did not show any growth of microorganism. Conclusion: Microbiological profile of pre-debridement, post-debridement, and surgical wound infection cultures of open fracture were different.