Phytohormones are small molecules present in very low concentrations in plants and provide a promising strategy to improve mass culture biotechnology due to their intrinsic role in growth, development, and survival. This study is aimed at evaluating the effect of different plant growth regulators on in vitro callus induction and shoot regeneration in tea. The experiment was conducted at Complete Randomize Design (CRD) with three replications. Callus induction and shoot regeneration was done by using Murashige & Skoog (MS) media supplemented with various concentrations and combinations of Naphthalene Acetic Acid (NAA), Benzyl Adenine (BA), and 2,4-Dichlorophenoxyacetic Acid (2,4-D). Shoot tips and nodal segments were used as explant. The highest percentage (86.51%) of callus induction was observed in the combined treatment of 2.00 milligram per litre (mg/L) BA + 1.50 mg/L 2, 4-D in 2.12 weeks. The same treatment produced the highest weight of calli, which was 0.18, 0.56, and 1.11 grams (g) for 6, 10, and 14 weeks after inoculation (WAI), respectively. The highest percentage (84.39%) of shoot induction was recorded with 2.00 mg/L BA + 2.00 mg/L NAA in 32.43 days. The maximum number (1.42, 2.46, and 4.49) and the highest length of the shoot (1.41, 3.28, and 4.61 cm) at 60, 90, and 120 DAI (days after inoculation) were also recorded from the same treatment. No callus or shoot induction was recorded in the controlled treatment. The protocol developed from the present study might be useful for the large-scale production of healthy, disease-free planting material for tea.