Nanoscale materials that are found in many kinds at nano scale level. Nanoparticles are vast class of things that contain small discrete mass of matter which have one particular range from 1-100nm (Laurent et al., 2008). They are may be zero dimension, two dimension and three dimension etc. The significance of these tools understands when researchers found that nano scale size is a property of this matter. Nanoparticles are complex molecules itself and it is formed from three layers (a) The outer most layer, which may be functionalized with a many biomolecules like proteins, DNA and other polymers and surfactants (b) The middle layer, which is chemically different substance from the inner in all characteristic, and (c) The inner, which is important the inner part of the NP and commonly known as nanoparticles (Shin, Cho, Kannan, Lee, & Kim, 2016)