During our investigation of Camellia sinensis diseases (2013-2018), a new leaf spot disease was found in seven provinces of China (Anhui, Fujian, Guangxi, Guizhou, Jiangxi, Tibet and Yunnan), occurring on both arboreal and terraced tea plants. The leaf spots were round to irregular, brown to dark brown, with grey or tangerine margins. Multi-locus (LSU, ITS, gapdh, tef-1α, tub2) phylogenetic analyses combined with morphological observations revealed four new species belonging to the genus Setophoma, i.e. S. antiqua, S. longinqua, S. yingyisheniae and S. yunnanensis. Of these four species, S. yingyisheniae was found to be present on diseased terraced tea plants in six of the seven sampled provinces (excluding Yunnan). The other three species only occurred on arboreal tea plants in Yunnan Province. In addition to the four species isolated from diseased leaves, S. endophytica sp. nov. was isolated from healthy leaves of terraced tea plants.