descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1988 English Publisher:Informa UK LimitedJournal:Connective Tissue Research, volume 17, pages 231-238 (issn: 0300-8207, eissn: 1607-8438,

Authors: Ryuichi Fujisawa; Yoshinori Kuboki;

doi: 10.3109/03008208809017474

pmid: 3208509

Increase of Dentin Phosphophoryn with Dentin Formation

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Abstract

Dentin phosphophoryn was quantified on bovine and rabbit dentin at three developmental stages. Phosphophoryn was extracted from teeth with 0.6M HCl, and quantified as optical density on DEAE-cellulose chromatogram or as phosphoserine content. Bovine phosphophoryn showed progressive increase with formation of dentin. Matrix-associated phosphophoryn was also quantified as phosphoserine content in insoluble dentin residue which was extracted with 6 M urea after decalcification. This fraction increased with formation of dentin both in bovine and rabbit dentin. Phosphophoryn is thought to be related to the later stage of dentin formation.

Related Organizations

Hokkaido Bunkyo University
Japan
Hokkaido University
Japan

Keywords

Incisor, Animals, Sodium Dodecyl Sulfate, Cattle, Electrophoresis, Polyacrylamide Gel, Rabbits, Dentinogenesis, Phosphoproteins, Chromatography, DEAE-Cellulose

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