Biomarker in tissue and serum plays significant roles in patient management and treatment and can be used as prognostic factors. For the diagnosis, treatment, prognosis study of breast cancer, we examined the expressions of Hypoxia inducible factor 1α (HIF-1α) in 128 cases mammary carcinoma tissues, and 43 cases serum levels of Cancer antigens153 (CA153), Cancer antigens125 (CA125) and CEA before and after neoadjuvant chemotherapy and analyze their relationship with clinicopathological factors such as metastasis, recurrence and chemotherapy. Our results demonstrated that the positive rates of HIF-1α were 68.75%, which were higher than that in DCIS, ADH, UDH (P 5cm) (P>0.05). Before and after therapy, the expression of HIF-1α had decreased significantly (P<0.05). The chemosensitivity of HIF-1α, CA153, and CEA positive group were worse than those of nagetive groups (P<0.05), however, there was no significant difference of chemosensitivity of CA125 before and after neoadjuvant chemotherapy. Biomarkers such as HIF-1α can be used as prognostic predictors in many malignancy and CA153 and CEA can be used as followed up potential factors of breast cancer chemosensitivity to help following up undergoing individualized chemotherapy.