ABSTRACT The mosquito fauna in urban parks in the city of São Paulo, Brazil, was investigated and compared for richness and diversity, and the abundance of each species was associated with climatic variables. Simultaneously, a virological investigation was performed to test the presence of Flavivirus and Alphavirus. Aspirations of adult mosquitoes were conducted in 3 urban parks for 3 consecutive weeks of each season between October 2018 and January 2020. A total of 2,388 mosquitoes were identified, with Culex quinquefasciatus, Cx. nigripalpus, and Aedes aegypti being the most abundant species. Mosquito assemblages showed similar richness and diversity, showing variability in individual results. Temperatures and Ae. aegypti abundance correlated significantly in one of the parks investigated herein. Urban parks represent areas of shelter and refuge for both anthropophilic and opportunistic species, such as Cx. quinquefasciatus and Ae. aegypti, as well as species that still need moderately preserved environments to develop.