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Revista Mexicana de Ciencias Forestales
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Revista Mexicana de Ciencias Forestales
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Rompimiento de la dormancia en semillas y propagación in vitro de Cordia elaeagnoides A. DC.

Authors: Fernando Santacruz Ruvalcaba; José Juvencio Castañeda Nava; Ana María Gaspar Peralta; Nancy Núñez Sandoval; Antonio Mora Santacruz;

Rompimiento de la dormancia en semillas y propagación in vitro de Cordia elaeagnoides A. DC.

Abstract

Cordia elaeagnoides es una especie maderable de selvas bajas caducifolias, que por la dificultad de sus semillas para germinar, la especie tiene limitaciones para reproducirse. En este contexto, se desarrolló un método para romper la dormancia y propagarla asexualmente por medio de proliferación de yemas axilares in vitro. Se evaluó la presencia de embriones sanos en 1 100 semillas de 1 lotes (100 semillas por lote); se constató que 39.45 % del total corresponden a esta condición. Se probaron 260 ppm de ácido giberélico con inmersión durante 24, 48 y 72 h; la primera alcanzó 96 % de germinación. Para establecer la propagación in vitro, se hizo rescate de embriones en el medio de cultivo Murashige y Skoog (MS) adicionado con 2 mgL-1 de benciladenina; se logró 82 % de germinación. Las plántulas obtenidas se utilizaron para la proliferación de yemas axilares, sobre las cuales se evaluaron los medios MS y McCown’s, y los reguladores de crecimiento 2-isopentiladenina, cinetina y benciladenina en dosis de 1, 2 y 3 mgL-1; se obtuvo un promedio de 3.03 brotes por explante tanto en cinetina como benciladenina. Para la rizogénesis in vitro se utilizó ácido 3-indolbutírico a dosis de 2.5 y 5 mgL-1, más adenina en oscuridad o fotoperiodo; los datos revelaron que cualquier dosis del ácido 3-indolbutírico induce la rizogénesis. Se concluye que para la germinación de semillas de Cordia elaeagnoides es conveniente aplicar ácido giberélico para eliminar la dormancia y para la propagación asexual in vitro, cinetina y benciladenina en las proporciones indicadas.

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Keywords

cordia elaeagnoides a. dc., Forestry, rizogénesis, rizogénesis, ácido giberélico, SD1-669.5, proliferación de yemas axilares, germinación, germinación, Environmental technology. Sanitary engineering, proliferación de yemas axilares, Agrociencias, Ácido giberélico, TD1-1066, Cordia elaeagnoides A. DC., rescate de embriones

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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