Filamentous hemagglutinin adhesin FhaB limits  i A baumannii  i biofilm formation

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2017 English Publisher:IMR PressJournal:Frontiers in Bioscience, volume 9, pages 266-275 (issn: 1945-0494, eissn: 1945-0508,

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Authors: Shakiba, Darwish Alipour Astaneh; Iraj, Rasooli; Seyed Latif, Mousavi Gargari;

doi: 10.2741/e801

pmid: 28199190

Filamentous hemagglutinin adhesin FhaB limits i A baumannii i biofilm formation

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Abstract

Increased resistance and survival, as well as immune evasion, play a significant role in the pathogenicity of Acinetobacter baumannii. Here, we report on the adhesion of the bacterium to epithelial cells and formation of biofilm on abiotic surfaces. We identificed autotransporter (AT) genes that encode homologues (Fha-like) of the two-partner secretion system (TPS) in A.baumannii (ATCC19606) which we designate as FhaB (exoprotein) and FhaC (transporter) and demonstrate that these novel genes, are under the control of distinct regulatable promoters within either the same (FhaBC) or two distinct (FhaB and FhaC) cells. The expression of this gene in outer membrane protein (OM) showed them to be deficient in the adherence to A546 cells. FhaB is involved in hydrophobicity of A. baumannii ATCC19606 while FhaBC is associated with bioﬁlm formation. The vaccinogenic potential of FHA-like proteins offers use of these targets as novel therapeutic strategies to limit A.baumannii associated morbidity and mortality.

Related Organizations

Semnan University
Iran (Islamic Republic of)
Shahed University
Iran (Islamic Republic of)

Keywords

Acinetobacter baumannii, Recombinant Fusion Proteins, Computational Biology, Gene Expression Regulation, Bacterial, Bacterial Adhesion, Biofilms, Escherichia coli, Amino Acid Sequence, Cloning, Molecular, Adhesins, Bacterial, Hydrophobic and Hydrophilic Interactions, Sequence Alignment, Conserved Sequence

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