The purpose of this study was to examine the effects of two methodologies (start method and sprocket size) of the Wingate Anaerobic Test (WAnT) on peak power (PP) and mean power (MP). Twenty power-trained males (24.6 ± 4.5 years; 25.4 ± 2.5 BMI) with the same exercise routine for the past 4 months performed, in a randomized order, four WAnT with different combinations of start method, flying (FLY) or stationary (STA), and front sprocket size, 62-tooth sprocket (S-62) or 85-tooth sprocket (S-85), using the Velotron Racermate®. The results showed main effects for start method (p 0.05; r = 0.227) or STA (p > 0.05; r = -0.132), and same sprocket size, S-62 (p > 0.05; r = -0.179), or S-85 (p > 0.05; r = 0.240). In conclusion, the findings of this study showed that FLY start and S-85 elicited higher means for PP and MP, respectively.Furthermore, Velotron with S-85 should not be interchangeably used with the S-62 model for power outputs; results suggest that different sprockets favored subjects differently, and no relationship can be established for the two methods. FLY start is suggested as a more effective method to reach elevated PP.